Assessment of biochemical parameters and characterization of TNFα −308G/A and PTPN22 +1858C/T gene polymorphisms in the risk of obesity in adolescents
| Autor: | María Antonia González-Zavala, Celia Nohemí Sánchez‑Domínguez, Hugo Leonid Gallardo‑Blanco, Miguel Ángel González-Madrazo, Sandra Cecilia Esparza González, Ana Cecilia Cepeda Nieto, Mauricio Andres Salinas‑Santander, Rafael Baltazar Reyes León‑Cachón |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
education.field_of_study Candidate gene business.industry General Neuroscience Insulin medicine.medical_treatment Population Articles General Medicine medicine.disease Obesity General Biochemistry Genetics and Molecular Biology PTPN22 03 medical and health sciences 030104 developmental biology Insulin resistance Genotype Immunology medicine Homeostatic model assessment General Pharmacology Toxicology and Pharmaceutics education business |
| Zdroj: | Biomedical Reports. 4:107-111 |
| ISSN: | 2049-9442 2049-9434 |
| DOI: | 10.3892/br.2015.534 |
| Popis: | Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα −308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα −308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα −308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed. |
| Databáze: | OpenAIRE |
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