Genomic and physiological analyses of an indigenous strain, Enterococcus faecium 17OM39
| Autor: | Vikas C. Ghattargi, Yogesh Nimonkar, Dimple Davray, Swapnil P. Doijad, Shreyas V. Kumbhare, Bhimashankar Utage, Bharati S. Meti, Shaunak A. Burse, Sudarshan A. Shetty, Om Prakash Sharma, Yogesh S. Shouche, Meghana A. Gaikwad, Shrikant Pawar, Mangesh V. Suryavanshi |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Enterococcus faecium 030106 microbiology Virulence Biology Hemolysis Microbiology law.invention Probiotic genes Feces 03 medical and health sciences Probiotic Plasmid Bacterial Proteins law Microbiologie RNA Ribosomal 16S Genetics Humans Microbiome Serum resistance Strain (chemistry) Probiotics Polysaccharides Bacterial Proteolytic enzymes Salt Tolerance General Medicine Genome analysis Bile salt hydrolysis biology.organism_classification 030104 developmental biology Indigenous probiotic HT29 Cells Genome Bacterial Bacteria Peptide Hydrolases |
| Zdroj: | Functional and Integrative Genomics, 18(4), 385-399 Functional and Integrative Genomics 18 (2018) 4 |
| ISSN: | 1438-793X |
| DOI: | 10.1007/s10142-018-0596-x |
| Popis: | The human gut microbiome plays a crucial role in human health and efforts need to be done for cultivation and characterisation of bacteria with potential health benefits. Here, we isolated a bacterium from a healthy Indian adult faeces and investigated its potential as probiotic. The cultured bacterial strain 17OM39 was identified as Enterococcus faecium by 16S rRNA gene sequencing. The strain 17OM39 exhibited tolerance to acidic pH, showed antimicrobial activity and displayed strong cell surface traits such as hydrophobicity and autoaggregation capacity. The strain was able to tolerate bile salts and showed bile salt hydrolytic (BSH) activity, exopolysaccharide production and adherence to human HT-29 cell line. Importantly, partial haemolytic activity was detected and the strain was susceptible to the human serum. Genomics investigation of strain 17OM39 revealed the presence of diverse genes encoding for proteolytic enzymes, stress response systems and the ability to produce essential amino acids, vitamins and antimicrobial compound Bacteriocin-A. No virulence factors and plasmids were found in this genome of the strain 17OM39. Collectively, these physiological and genomic features of 17OM39 confirm the potential of this strain as a candidate probiotic. |
| Databáze: | OpenAIRE |
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