Bovine amelogenin message heterogeneity: alternative splicing and Y-chromosomal gene transcription
Autor: | Carolyn W. Gibson, William R. Abrams, G Shen, Ellis E. Golub, J. Rosenbloom, Wendi Ding |
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Rok vydání: | 1992 |
Předmět: |
Male
X Chromosome Transcription Genetic Protein Conformation RNA Splicing Molecular Sequence Data Restriction Mapping Biology Polymerase Chain Reaction Biochemistry Protein structure Dental Enamel Proteins stomatognathic system Sequence Homology Nucleic Acid Y Chromosome Complementary DNA Animals Humans Amino Acid Sequence RNA Messenger Peptide sequence chemistry.chemical_classification Genomic Library Amelogenin Base Sequence Alternative splicing Nucleic acid sequence Tooth Germ Translation (biology) DNA Exons Molecular biology Amino acid chemistry Cattle |
Zdroj: | Biochemistry. 31:8384-8388 |
ISSN: | 1520-4995 0006-2960 |
Popis: | The amelogenins are the most abundant proteins in developing tooth enamel. Previous analyses have demonstrated that transcriptionally active genes encoding the proteins are located on both the bovine X and the bovine Y chromosomes. We report here the cloning and sequence analysis of the Y-chromosomal gene and corresponding cDNA. The Y-specific mRNA encodes a translation product in which a 21 amino acid domain has been deleted, relative to the X-specific amelogenin, resulting in loss of a structure tentatively described as a beta-spiral. There are also 13 single amino acid differences compared to the X-specific amelogenin. In addition, we have cloned and sequenced an X-chromosomal alternatively spliced amelogenin cDNA that encodes a 43 amino acid amelogenin primary translation product. Hydrophobicity analysis indicates that all analyzed amelogenin proteins have a mean hydrophilic character and the two peptides translated from alternatively spliced messages have significant increases in percentage of hydrophobic amino acids. |
Databáze: | OpenAIRE |
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