Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
| Autor: | Carolyn J. Hovde, Rodolfo Juan Carlos Cantet, E.C. Mercado, Ángel Adrián Cataldi, Daniel A. Vilte, Cristina Ibarra, Luisina Martorelli, Adriana Andrea Albanese, Elsa Zotta |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Biotecnología relacionada con la Salud lcsh:Medicine Calves medicine.disease_cause fluids and secretions Colonization purl.org/becyt/ford/3.4 [https] Experimental infection Pathogen Escherichia coli Infections Bacterial Shedding 2. Zero hunger 0303 health sciences HAEMOLYTIC UREMIC SYNDROME biology Escherichia coli Proteins General Medicine Antibodies Bacterial 3. Good health Host-Pathogen Interactions BACTERIA purl.org/becyt/ford/3 [https] Antibody Research Article Locus of enterocyte effacement CIENCIAS MÉDICAS Y DE LA SALUD Article Subject Infection Dose Cattle Diseases H7 [E. coli O157] Escherichia coli O157 General Biochemistry Genetics and Molecular Biology Microbiology Biotecnología de la Salud 03 medical and health sciences Antibodies anti-LEE Immune system medicine Animals Escherichia coli 030304 developmental biology General Immunology and Microbiology 030306 microbiology lcsh:R Phosphoproteins biology.organism_classification Disease Models Animal Immunology biology.protein Cattle ESCHERICHIA COLI Bacteria |
| Zdroj: | BioMed Research International Vol.2015 (2015) FAUBA Digital (UBA-FAUBA) Universidad de Buenos Aires. Facultad de Agronomía instacron:UBA-FAUBA Vol.2015 Article ID 290679 BioMed Research International, Vol 2015 (2015) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | Escherichia coli O157:H7 is the main causative agent of haemolytic uremic syndrome. Cattle are the main reservoir of these bacteria, and have been shown to develop immune response to colonization. Our aim was to investigate the faecal shedding pattern of E. coli O157:H7 in calves challenged intragastrically with either 108 or 1010 CFU, as well as the ability of specific preexisting antibodies to reduce shedding of the pathogen. Shedding was analysed by direct counting as well as enrichment of rectoanal mucosal swabs. Statistical analysis was performed using a linear model for repeated measures with and without the inclusion of preexisting antibodies against the carboxy-terminal fraction of intimin- (-intimin C280) as a covariable. Results suggest that there is a statistical difference in the area under the shedding curves between both doses for 14 as well as 28 days after challenge (p = 0.0069 and 0.0209, resp.). This difference is increased when the prechallenge antibodies are taken into account (p = 0.0056 and 0.0185). We concluded that the bacterial dose influences shedding on calves experimentally challenged and that preexisting antibodies against E. coli O157:H7 -intimin C280 could partially reduce faecal excretion. Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hovde, C. J.. University Of Idaho; Estados Unidos Fil: Vilte, D. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina Fil: Albanese, A.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mercado, E. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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