Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat
| Autor: | A. I. Speranskii, E. A. Kalashnikova, T. A. Ryazantseva, N. N. Veiko, A. V. Ermakov, Svetlana V. Kostyuk, O. A. Kupavtseva |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Serum chemistry.chemical_classification biology medicine.diagnostic_test General Medicine Ribosomal RNA DNA Ribosomal Molecular biology General Biochemistry Genetics and Molecular Biology Epitope chemistry.chemical_compound Blood serum Enzyme chemistry Antibodies Antinuclear Immunoassay biology.protein medicine Animals Humans Female Antibody Ribosomal DNA DNA |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 144:304-308 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | Enzyme immunoassay showed that blood serum from healthy donors contains specific high-affinity antibodies (apparent association constant > or = 5 x 10(9) M(-1)) against a fragment of transcribed region of ribosomal DNA repeat of human serum, which are present in a free form or are bound to extracellular DNA. Preheating of the serum at 55 degrees C and high ionic strength (1.5 M NaCl) had no effect on the interaction of antibodies with this fragment. Competitive binding assay showed that these antibodies recognize DNA epitopes, which differ from the epitopes recognized by most anti-DNA antibodies in systemic lupus erythematosus. |
| Databáze: | OpenAIRE |
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