Resolvin D1 regulates epithelial ion transport and inflammation in cystic fibrosis airways

Autor: Gerard Higgins, Isabelle Sermet-Gaudelus, Paul McNally, Monika Hollenhorst, Ahmad Moukachar, Aurélie Hatton, Coral Fustero-Torre, Valerie Urbach, Fiona C. Ringholz, Brian J. Harvey, Ali Sassi
Přispěvatelé: Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Rok vydání: 2018
Předmět:
0301 basic medicine
Pulmonary and Respiratory Medicine
Cystic Fibrosis
Docosahexaenoic Acids
Inflammation
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Cystic fibrosis
Cell Line
Pathogenesis
Mice
03 medical and health sciences
0302 clinical medicine
Mediator
In vivo
Macrophages
Alveolar
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
medicine
Animals
Humans
Secretion
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Child
Cells
Cultured
ComputingMilieux_MISCELLANEOUS
Ion Transport
business.industry
Epithelial Cells
respiratory system
medicine.disease
respiratory tract diseases
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
030104 developmental biology
030220 oncology & carcinogenesis
Pediatrics
Perinatology and Child Health
Immunology
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Tumor necrosis factor alpha
medicine.symptom
business
Ex vivo
Zdroj: Journal of Cystic Fibrosis
Journal of Cystic Fibrosis, Elsevier, 2018, 17 (5), pp.607-615. ⟨10.1016/j.jcf.2017.11.017⟩
ISSN: 1569-1993
DOI: 10.1016/j.jcf.2017.11.017
Popis: Background Cystic Fibrosis (CF) lung disease is characterised by dysregulated ion transport that promotes chronic bacterial infection and inflammation. The impact of the specialised pro-resolution mediator resolvin D1 (RvD1) on airway surface liquid (ASL) dynamics and innate defence had not yet been investigated in CF airways. Methods Ex vivo studies were performed on primary cultures of alveolar macrophages and bronchial epithelial cells from children with CF and in human bronchial epithelial cell lines; in vivo studies were performed in homozygous F508del-CFTR mice treated with vehicle control or RvD1 (1–100nM). Results RvD1 increased the CF ASL height in human bronchial epithelium and restored the nasal trans-epithelial potential difference in CF mice by decreasing the amiloride-sensitive Na + absorption and stimulating CFTR-independent Cl − secretion. RvD1 decreased TNFα induced IL-8 secretion and enhanced the phagocytic and bacterial killing capacity of human CF alveolar macrophages. Conclusion RvD1 resolves CF airway pathogenesis and has therapeutic potential in CF lung disease.
Databáze: OpenAIRE
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