Comparative Evaluation of a Novel Phage Protein Ligand Assay and Immunomagnetic Separation Method To Isolate the Seven Top Serogroups of Escherichia coli (O157, O26, O103, O145, O111, O45, and O121) in Foods at Risk
| Autor: | S. D. Miszczycha, M. Bouvier, D. Thevenot Sergentet, Charlène Tadla, L. Collinet, X. Guillarme |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Meat 030106 microbiology Serogroup medicine.disease_cause Immunomagnetic separation Microbiology law.invention 03 medical and health sciences law medicine Humans Bacteriophages Escherichia coli Shiga-Toxigenic Escherichia coli biology Immunomagnetic Separation business.industry Pathogenic bacteria Contamination Food safety biology.organism_classification Isolation (microbiology) Food Microbiology Recombinant DNA business Bacteria Food Science |
| Zdroj: | Journal of Food Protection. 80:1973-1979 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x.jfp-16-479 |
| Popis: | The presence of Shiga toxin-producing Escherichia coli (STEC) in food is a major concern for food safety authorities and industries. Methods for detecting these pathogenic bacteria are crucial. Enrichment of foods for STEC identification has been optimized, but selective concentration of bacteria before isolation still needs to be improved. In the present study, we tested the performance of the VIDAS ESPT detection method against that of the immunomagnetic separation (IMS) method. A preenrichment inoculation was performed to provide a realistic scenario of the contamination that occurs in foods, and the methods were then compared. Results obtained were then confirmed in naturally contaminated foods. Preenrichment inoculation assays revealed that the novel concentration method using phage recombinant proteins or the selective capture of the target top seven STEC serogroups is as specific and sensitive as IMS. Subsequent evaluation of naturally contaminated samples confirmed that the novel concentration method and IMS are equivalent in performance under the conditions tested. |
| Databáze: | OpenAIRE |
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