Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa
| Autor: | Xiangqin Wang, Xu Wang, Yong-Ding Liu, Fayu Zhang, Hong Zhang, Zengliang Yu, Xiang Xiao, Qing Huang, Chunxiang Hu |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Chlorophyll a
biology Pseudomonas putida fungi Aquatic Science biology.organism_classification Photosynthesis 16S ribosomal RNA Cyanobacterial blooms Microbiology chemistry.chemical_compound chemistry Biological control Phytoplankton Phycocyanin Microcystis aeruginosa Phytoplankton-lytic bacterium Bacteria |
| Zdroj: | Limnologica. 41:70-77 |
| ISSN: | 0075-9511 |
| Popis: | A bacterial strain named CH-22 showing phytoplankton-lytic activity against bloom-forming cyanobacterium Microcystis aeruginosa was isolated from Lake Chaohu of Anhui Province, China. The isolated strain was identified as Pseudomonas putida by morphology and homology research based on 16S rDNA. The phytoplankton-lytic activity was confirmed by reduction in cell number and chlorophyll a concentration of M. aeruginosa in an infected culture for a defined period. The initial bacterial and M. aeruginosa densities affected the phytoplankton-lytic activity significantly. When the 15% (150 mu L/mL) concentration of bacterial cultures was infected, the highest phytoplankton-lytic activity reached to 98.8% after 7 days. When the initial M. aeruginosa density was less than 3 x 10(6) cells/mL, about 90.0% of chlorophyll a was removed. Obvious reduction in phycocyanin concentration in the treated M. aeruginosa suggests that isolated strain may possibly inhibit the synthesis of photosynthetic apparatus. Supernatants of bacterial cultures showed higher phytoplankton-lytic activity, suggesting that phytoplankton-lytic bacterium P. putida indirectly attacked M. aeruginosa cells by secretion of extracellular antialgal substances, which is characterized as anti-heat shock. The isolated P. putida also showed effective phytoplankton-lytic activity against a wide range of phytoplankton. These results suggest that indigenous bacteria isolated from eutrophic lake may be employed to regulate the ecological balance between the phytoplankton and bacteria, and consequently, to reduce the occurrence of cyanobacterial blooms in freshwaters. (C) 2010 Elsevier GmbH. All rights reserved. |
| Databáze: | OpenAIRE |
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