Characteristics of Raw Starch-Digesting α-Amylase of Streptomyces badius DB-1 with Transglycosylation Activity and Its Applications
| Autor: | L. Shivlata, Tulasi Satyanarayana |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glycosylation Starch Cost-Benefit Analysis Statistics as Topic Oligosaccharides Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences Hydrolysis chemistry.chemical_compound 010608 biotechnology Enzyme Stability Amylase Maltose Molecular Biology chemistry.chemical_classification Molecular mass biology Temperature Bread General Medicine Hydrogen-Ion Concentration Streptomyces Moderate temperature Molecular Weight Kinetics 030104 developmental biology Enzyme Monomer chemistry Batch Cell Culture Techniques Metals Fermentation biology.protein Streptomyces badius alpha-Amylases Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 181:1283-1303 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-016-2284-4 |
| Popis: | Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL−1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The purified enzyme is a monomer with a molecular mass of ~57 kDa and optimally active at 50 °C and pH 6.0. The enzyme hydrolyzes soluble as well as raw starches into simpler sugars with a high proportion (>40.0 %) of maltotetraose. It is optimally active at moderate temperature and generates maltooligosaccharides from starch, thus, useful as an antistale in bread making. It also plays a role in increasing the formation of maltooligosaccharides due to transglycosylation activity, thus, finds application in functional foods. This is the first report on the production of raw starch-digesting α-amylase by S. badius with transglycosylation activity. |
| Databáze: | OpenAIRE |
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