Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens
| Autor: | Daniel R. Kuritzkes, Anupriya Singh, Mohammadali Safavieh, Hadi Shafiee, Karan Dhingra, Adnan Memic, Vivasvat Kaul, Mohamed Shehata Draz, Sultan Khetani, Manoj Kumar Kanakasabapathy |
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| Rok vydání: | 2017 |
| Předmět: |
Paper
Silver Materials science Loop-mediated isothermal amplification Nanotechnology 02 engineering and technology Sensitivity and Specificity 01 natural sciences Article Nanocomposites law.invention law Lab-On-A-Chip Devices General Materials Science Electrodes DNA Primers Graphene 010401 analytical chemistry Silver Nano Nucleic acid amplification technique Amplicon 021001 nanoscience & nanotechnology 0104 chemical sciences 3. Good health Viral replication HIV-1 Nucleic acid RNA Viral Graphite 0210 nano-technology Nucleic Acid Amplification Techniques Viral load |
| Zdroj: | Nanoscale. 9:1852-1861 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c6nr06417e |
| Popis: | Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 106 and 108 copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg µl−1 and 108 fg µl−1. The target HIV-1 nucleic acid was amplified using the RT-LAMP technique and detected through the electrical sensing of LAMP amplicons for a broad range of RNA concentrations between 10 fg µl−1 and 108 fg µl−1 after 40 min of amplification time. Our assay may be used for antiretroviral therapy monitoring where it meets the sensitivity requirement of the World Health Organization guidelines. Such a paper microchip assay without the amplification step may also be considered as a simple and inexpensive approach for acute HIV detection where maximum viral replication occurs. |
| Databáze: | OpenAIRE |
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