Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10

Autor: Jan van der Ploeg, Dick B. Janssen
Přispěvatelé: Groningen Biomolecular Sciences and Biotechnology
Rok vydání: 1995
Předmět:
EXPRESSION
Environmental Engineering
Transcription
Genetic
Sequence analysis
Hydrolases
Molecular Sequence Data
PROTEIN
BIODEGRADATION
Bioengineering
Biology
Microbiology
Homology (biology)
CLONING
PSEUDOMONAS-PUTIDA PP3
Open Reading Frames
Bacterial Proteins
SIGMA(54)-DEPENDENT REGULATION
Transcription (biology)
NUCLEOTIDE-SEQUENCE
Sequence Homology
Nucleic Acid
Gene expression
ELEMENTS
Escherichia coli
Environmental Chemistry
2-DICHLOROETHANE
Amino Acid Sequence
Gene
Dehalogenase
Genetics
Base Sequence
Gram-Negative Aerobic Bacteria
Symporters
HALOACETATE
Nucleic acid sequence
Biological Transport
Gene Expression Regulation
Bacterial
Sequence Analysis
DNA
DEGRADATION
Pollution
Open reading frame
ESCHERICHIA-COLI
Genes
Bacterial
RHIZOBIUM-MELILOTI
Carrier Proteins
1
2-DICHLOROETHANE
Transcription Factors
Zdroj: Biodegradation, 6(3), 257-263
ISSN: 0923-9820
Popis: The DNA sequence upstream of the dhlB gene encoding the haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10 was determined and contained an open reading frame, designated dhlC, which encoded a protein with a significant similarity with the family of Na+-dependent symport proteins. The dhlC gene was subcloned under control of a T7 promoter, and found to encode a polypeptide of 45 kDa on SDS-PAGE. Upstream of dhlC, a -24/-12 promoter sequence was found. Further upstream, in the opposite direction of transcription, another open reading frame, designated dhlR,with homology with the family of sigma(54)-dependent transcriptional activator proteins was detected. The dhlR gene was cloned and expressed under the control of a T7 promoter and encoded a polypeptide of 51 kDa on SDS-PAGE. The genetic organization of the dhlB region suggested that the expression of dhlC and dhlB was controlled by the product of dhlR and sigma(54) which may explain the observed overexpression of the haloalkanoic acid dehalogenase under starvation conditions.
Databáze: OpenAIRE
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