Global DNA methylation levels in white blood cells of patients with chronic heroin use disorder. A prospective study
| Autor: | Chiung-Wen Hu, Kakia Nikolaou, Leda Kovatsi, Mu-Rong Chao, Domniki Fragou |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Health Toxicology and Mutagenesis media_common.quotation_subject Opioid 010501 environmental sciences Toxicology 01 natural sciences Heroin 03 medical and health sciences 0302 clinical medicine RA1190-1270 Internal medicine medicine Epigenetics Prospective cohort study ComputingMethodologies_COMPUTERGRAPHICS 0105 earth and related environmental sciences media_common DNA methylation Abstinence business.industry Regular Article Methylation Toxicology. Poisons Inclusion and exclusion criteria Detoxification business Drug use disorder 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Toxicology Reports, Vol 8, Iss, Pp 337-342 (2021) Toxicology Reports |
| ISSN: | 2214-7500 |
| DOI: | 10.1016/j.toxrep.2021.02.006 |
| Popis: | Graphical abstract Highlights • Opioid abstinence for 21 days does not affect global DNA methylation levels in white blood cells. • All participants in the study completed a 21-day “dry” detoxification program. • Findings do not rule out the possibility of site-specific methylation changes. Background Increasing scientific evidence shows the significant role of epigenetic mechanisms in drug use disorder, abstinence and relapse. Studies on human subjects are limited compared to those on animals, for various reasons such as poly-substance abuse, high drop-out rate and technical difficulties. Objectives Our goal was to evaluate whether a monitored abstinence period of 21 days could induce changes in global DNA methylation in chronic heroin users. Method In the current study, we present data on global DNA methylation on a set of 18 male patients with chronic heroin use disorder, carefully selected based on inclusion and exclusion criteria, who were hospitalized and closely monitored during a 21-day detoxification program, one of the few where no opioid agonist is administered. The participants were sampled twice, once upon enrolment to the program and once upon completion. Results According to our results, no difference in global DNA methylation was detected between samples collected upon enrolment and samples collected upon completion of the program. Conclusion The findings of this study do not rule out the possibility that the 21-day abstinence period was not long enough to observe changes in global DNA methylation, or that abstinence induced site-specific methylation changes (but not global changes), that certainly merit further evaluation. |
| Databáze: | OpenAIRE |
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