Western blot seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) in Fortaleza (Brazil): a serological and molecular diagnostic and epidemiological approach
| Autor: | Reinaldo B. Oriá, Francisca C F de Sousa, Anne-Mieke Vandamme, Ana C S Oliveira, Veralice Meireles Sales de Bruin, Francine Bovy de Castro Costa, Rosa Maria Salani Mota, Jan Desmyter, Anderson Gomes, Vania Barreto, Patrick Goubau, Sonia Van Doren, Carlos Maurício de Castro Costa, Anna B Carneiro-Proietti, Terezinha de Jesus Teixeira Santos |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Chagas disease medicine.medical_specialty Epidemiology Cross-sectional study lcsh:QR1-502 syphilis blood transfusion Biology seroindeterminate individuals lcsh:Microbiology lcsh:Infectious and parasitic diseases Serology medicine lcsh:RC109-216 hepatitis Hepatitis medicine.disease biology.organism_classification Virology PCR Infectious Diseases HTLV-1/2 Human T-lymphotropic virus 1 Immunology Human T-lymphotropic virus 2 Syphilis |
| Zdroj: | Brazilian Journal of Infectious Diseases v.7 n.3 2003 Brazilian Journal of Infectious Diseases Brazilian Society of Infectious Diseases (BSID) instacron:BSID Brazilian Journal of Infectious Diseases, Vol 7, Iss 3, Pp 202-209 (2003) Brazilian Journal of Infectious Diseases, Vol 7, Iss 3, Pp 202-209 Brazilian Journal of Infectious Diseases, Volume: 7, Issue: 3, Pages: 202-209, Published: JUN 2003 |
| ISSN: | 1413-8670 |
| DOI: | 10.1590/s1413-86702003000300005 |
| Popis: | How to handle Western blot (WB) seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) constitutes a challenge for blood banks and fam ilies. We made a cross-sectional study of 191 enzyme linked immunoassay (EIA) reactive individuals from the hematological center (HEMOCE) of Fortaleza (Brazil), examining their serological (WB) and molecular (PCR) diagnosis, and demographic profiles, as well as a possible association of their condition with other infectious pathologies and risk factors. Ethical institutional approval and personal consent were obtained. Out of 191 EIA reactive individuals, 118 were WB seroindeterminate and 73 were seropositive for HTLV-1/2. In the PCR analysis of 41 WB seroindeterminate individuals, 9 (22%) were positive and 32 (78%) were negative for HTLV-1/2. The demographic analysis indicated a trend towards a predominance of males among the seroindeterminate individuals and females in the seropositive ones. The seroindeterminate individuals were younger than the seropositive ones. We did not find any association of these conditions with syphilis, Chagas disease or HIV or hepatitis, and with risk factors such as breast-feeding, blood transfusion, STD (syphilis) and IDU. |
| Databáze: | OpenAIRE |
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