Impact of methylene blue in addition to norepinephrine on the intestinal microcirculation in experimental septic shock
| Autor: | Jordan Nantais, Tristan C. Dumbarton, Alexander Maxan, Juan Zhou, Christian Lehmann, Samuel Minor, Nizam Farah |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Mean arterial pressure Endothelium Lipopolysaccharide Physiology Pharmacology Microcirculation Norepinephrine (medication) chemistry.chemical_compound Norepinephrine Physiology (medical) medicine Cell Adhesion Leukocytes Pressure Animals business.industry Septic shock Hemodynamics Hematology medicine.disease Shock Septic Rats Endotoxins Intestines Methylene Blue Disease Models Animal medicine.anatomical_structure chemistry Rats Inbred Lew Immunology Endothelium Vascular Cardiology and Cardiovascular Medicine business Intravital microscopy Methylene blue medicine.drug |
| Zdroj: | Clinical hemorheology and microcirculation. 58(1) |
| ISSN: | 1875-8622 |
| Popis: | Methylene blue (MB) has been used with some success as a treatment for the vasoplegia of vasopressor-refractory septic shock. The putative mechanism of action of MB is the inhibition of endothelial nitric oxide within the microvasculature and improved responsiveness to endogenous catecholamines (norepinephrine (NE)). However, to date, no study has demonstrated the microcirculatory effect of methylene blue in septic shock. The objective of this randomized, controlled, animal study was to show, in an experimentally-induced, septic shock model in rats, the effects of MB and NE on global hemodynamics and the microcirculation. Mean arterial pressure (MAP) was drastically reduced following bacterial endotoxin (lipopolysaccharide, LPS) administration in animals not receiving vasopressors. Only the combination of NE + MB restored MAP to control levels by the end of the three hour experiment. Intravital microscopy of the microcirculation was performed in the terminal ileum in order to examine functional capillary density in intestinal muscle layers and the mucosa, as well as leukocyte activation in venules (rolling, adhesion to the endothelium). Untreated LPS animals showed a significant increase in leukocyte adhesion and a decrease in capillary perfusion in the intestinal microcirculation. In groups receiving NE or NE+MB, we observed a significant decrease in leukocyte adhesion and improved functional capillary density, indicating that microvasculature function was improved. This study suggests that methylene blue may be able to improve hemodynamics while preserving microvascular function in septic shock. |
| Databáze: | OpenAIRE |
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