The application of near-infrared spectra micro-image in the imaging analysis of biology samples
| Autor: | Shun-Geng Min, Dong Wang, Zhong-Hua Guo, Yun-Sheng Ding |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Starch
principal component analysis Near-infrared spectra micro-image Biomedical Engineering Analytical chemistry Medicine (miscellaneous) Biology compound distribution lcsh:Technology chemistry.chemical_compound tobacco leaf Near infrared spectra lcsh:QC350-467 Food science plant glandular hair lcsh:T Near-infrared spectroscopy fungi food and beverages Fructose Carbohydrate Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Imaging analysis Tissue sections chemistry Principal component analysis lcsh:Optics. Light |
| Zdroj: | Journal of Innovative Optical Health Sciences, Vol 7, Iss 4, Pp 1350062-1-1350062-10 (2014) |
| ISSN: | 1793-7205 1793-5458 |
| DOI: | 10.1142/S1793545813500624 |
| Popis: | In this research, suitable imaging methods were used for acquiring single compound images of biology samples of chicken pectorales tissue section, tobacco dry leaf, fresh leaf and plant glandular hair, respectively. The adverse effects caused by the high water content and the thermal effect of near infrared (NIR) light were effectively solved during the experiment procedures and the data processing. PCA algorithm was applied to the NIR micro-image of chicken pectorales tissue. Comparing the loading vector of PC3 with the NIR spectrum of dry albumen, the information of PC3 was confirmed to be provided mainly by protein, i.e., the 3rd score image represents the distribution trend of protein mainly. PCA algorithm was applied to the NIR micro-image of tobacco dry leaf. The information of PC2 was confirmed to be provided by carbohydrate including starch mainly. Compared to the 2nd score image of tobacco dry leaf, the compared correlation image with the reference spectrum of starch had the same distribution trend as the 2nd score image. The comparative correlation images with the reference spectra of protein, glucose, fructose and the total plant alkaloid were acquired to confirm the distribution trend of these compounds in tobacco dry leaf respectively. Comparative correlation images of fresh leaf with the reference spectra of protein, starch, fructose, glucose and water were acquired to confirm the distribution trend of these compounds in fresh leaf. Chemimap imaging of plant glandular hair was acquired to show the tubular structure clearly. |
| Databáze: | OpenAIRE |
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