Smooth muscle contractility in prostatic hyperplasia: Role of cyclic adenosine monophosphate
| Autor: | P. Drescher, R. E. Eckert, Paul O. Madsen |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Adenosine monophosphate medicine.medical_specialty Pyridones Urology Prostatic Hyperplasia Contractility Phenylephrine chemistry.chemical_compound Theophylline Papaverine Internal medicine Cyclic AMP medicine Humans Cyclic adenosine monophosphate Forskolin Dose-Response Relationship Drug business.industry Colforsin Phosphodiesterase Muscle Smooth Smooth muscle contraction Endocrinology Bucladesine Oncology chemistry Milrinone business Muscle Contraction medicine.drug |
| Zdroj: | The Prostate. 25:76-80 |
| ISSN: | 1097-0045 0270-4137 |
| DOI: | 10.1002/pros.2990250204 |
| Popis: | The role of cyclic 3'-5' adenosine monophosphate (cAMP) on alpha 1-adrenoceptor (alpha 1-receptor) induced smooth muscle contractions in symptomatic benign prostatic hyperplasia (BPH) was investigated. Application of the selective alpha 1-receptor agonist phenylephrine (PE) induced fully reversible contractions in a dose-dependent fashion. Phosphodiesterase (PDE) inhibitors blocking the degradation of cAMP suppressed the PE induced contractions as follows: theophylline (1 mM), 91.1 +/- 1.4%; papaverine (0.5 mM), 822.8 +/- 3.2%; milrinone (0.5 mM), 68.2 +/- 0.6%. Forskolin (50 microM), which elevates cAMP through direct activation of adenylatecyclase (AC), inhibited the PE induced contractions by 82.4 +/- 3.6%. To further increase the intracellular cAMP concentration ([cAMP]i), the membrane permeable cAMP analogue N6-2'-O-dibutyryladenosine derivative (dBcAMP; 1 mM) was applied and reduced the PE evoked contractions by 69.8 +/- 2.3%. We conclude that elevation of [cAMP]i is an important step in inducing smooth muscle relaxation. |
| Databáze: | OpenAIRE |
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