A systematic investigation of the bio-toxicity of core-shell magnetic mesoporous silica microspheres using zebrafish model
| Autor: | Ahmed A. Elzatahry, Kholoud E. Albinali, Yu Zhang, Hesham M. Ismail, Areej Abdulkareem Al-Khalaf, Rafael M. Prieto, Moustafa M. Zagho, Yonghui Deng, Gheyath K. Nasrallah |
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| Rok vydání: | 2018 |
| Předmět: |
02 engineering and technology
010402 general chemistry 01 natural sciences Microsphere Core shell Magnetic nanomaterials Teratogenicity Neurotoxicity General Materials Science Zebrafish Core-shell microspheres Acute toxicity biology Chemistry General Chemistry Mesoporous silica 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 0104 chemical sciences Mechanics of Materials Toxicity Drug delivery 0210 nano-technology Mesoporous material Nuclear chemistry |
| Zdroj: | Microporous and Mesoporous Materials. 265:195-201 |
| ISSN: | 1387-1811 |
| DOI: | 10.1016/j.micromeso.2018.02.008 |
| Popis: | In this work, shearing interface coassembly in biliquid phase systems is employed to synthesize biocompatible core-shell magnetic mesoporous silica microspheres with uniform size of about 600 nm, perpendicular mesopores of 6.0 nm and large pore volume of 0.77 cm3/g. The toxicology assays based on the zebrafish model was conducted to test under a high-throughput manner for the biosafety of Fe3O4@RF@mSiO2 microspheres. The highest no observed toxic effect concentration (NOEC) estimated by the acute toxicity assay for the microspheres was 1.6 mg/mL. The estimated number (measured by ICP-MS) of the penetrated microspheres at this concentration was 2 × 106 per embryo. The results of three different performed toxicity assays show no overall acute toxicity, teratogenicity, or neurotoxicity of the microspheres on zebrafish embryos at any of the tested concentrations (from 0.1 to 1.6 mg/mL) via its multifunctional microstructure. Here, gemcitabine (GEM) as a model of anti-cancer drug was loaded into the mesopores of Fe3O4@RF@mSiO2 microspheres to study their drug release behavior. The microspheres were found to exhibit pH responsive property, which benefits for the GEM release under cancer therapy. Overall, this study offers promising avenue for effective evaluation of magnetic core-shell microspheres for drug delivery and cancer therapy applications. |
| Databáze: | OpenAIRE |
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