Detection of mutations in the VP7 gene of vaccine-derived strains shed by monovalent rotavirus vaccine recipients
| Autor: | Mana Inoue, Hiroshi Sakiyama, Sayaka Takanashi, Mei Kaneko, Masashi Mizuguchi, Hiroshi Ushijima, Shoko Okitsu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Short Communication 030106 microbiology Reversion Biology medicine.disease_cause Virus 03 medical and health sciences shedding 0302 clinical medicine Antigen Rotavirus vaccine medicine General Materials Science 030212 general & internal medicine chemistry.chemical_classification Mutation Virology Rotavirus vaccine Amino acid rotavirus chemistry VP7 gene mutation Vp7 gene |
| Zdroj: | Access Microbiology |
| ISSN: | 2516-8290 |
| Popis: | Strains of Rotarix, a live attenuated monovalent oral rotavirus vaccine, replicate in the intestine and are shed for about one month in immunocompetent recipients. The current study aimed to identify genetic changes of shed strains to reveal any significant mutations and their clinical impact on recipients. Stool samples of recipients of the first dose of Rotarix were sequentially collected for one month from the day of administration. Sequence analyses of the VP7 gene in eight recipients revealed five amino acid substitutions. Among them, two were observed in aa123, which is located in antigenic region 7-1a. Since there were no associated clinical symptoms, the genetic changes were unlikely to have caused reversion of pathogenicity of vaccine strain. Of interest, the virus in one case became closer to wild-type rotavirus via an amino acid change at aa123 occurring 14 days after administration, which might have resulted from multiple replications and long-term shedding of the vaccine strain. |
| Databáze: | OpenAIRE |
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