EST-SNP study of olea Europaea L. Uncovers functional polymorphisms between cultivated and wild olives
| Autor: | Lorenzo León, Angjelina Belaj, Luciana Baldoni, Soraya Mousavi, Federico Brizioli, Raúl de la Rosa, Roberto Mariotti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Genotype lcsh:QH426-470 Single-nucleotide polymorphism Subspecies subsp. guanchica Polymorphism Single Nucleotide 01 natural sciences Article functional markers Domestication 03 medical and health sciences Phylogenetics Olea Genetics cultivars Humans genomics-assisted breeding Genotyping Phylogeny Genetics (clinical) Expressed Sequence Tags Expressed sequence tag ubsp.guanchica oleasters EST-SNPs candidategenes conservation biology Olea europaea subsp. europaea Genetic Variation Genomics biology.organism_classification Plant Breeding lcsh:Genetics Olea europaeasubsp.europaea Genetics Population 030104 developmental biology EST–SNPs candidate genes 010606 plant biology & botany |
| Zdroj: | Genes (Basel) 11 (2020): 1–19. doi:10.3390/genes11080916 info:cnr-pdr/source/autori:Mariotti R.; Belaj A.; De La Rosa R.; Leon L.; Brizioli F.; Baldoni L.; Mousavi S./titolo:EST-SNP study of olea Europaea L. Uncovers functional polymorphisms between cultivated and wild olives/doi:10.3390%2Fgenes11080916/rivista:Genes (Basel)/anno:2020/pagina_da:1/pagina_a:19/intervallo_pagine:1–19/volume:11 Genes, Vol 11, Iss 916, p 916 (2020) Genes Volume 11 Issue 8 |
| DOI: | 10.3390/genes11080916 |
| Popis: | Background: The species Olea europaea includes cultivated varieties (subsp. europaea var. europaea), wild plants (subsp. europaea var. sylvestris), and five other subspecies spread over almost all continents. Single nucleotide polymorphisms in the expressed sequence tag able to underline intra-species differentiation are not yet identified, beyond a few plastidial markers. Methods: In the present work, more than 1000 transcript-specific SNP markers obtained by the genotyping of 260 individuals were studied. These genotypes included cultivated, oleasters, and samples of subspecies guanchica, and were analyzed in silico, in order to identify polymorphisms on key genes distinguishing different Olea europaea forms. Results: Phylogeny inference and principal coordinate analysis allowed to detect two distinct clusters, clearly separating wilds and guanchica samples from cultivated olives, meanwhile the structure analysis made possible to differentiate these three groups. Sequences carrying the polymorphisms that distinguished wild and cultivated olives were analyzed and annotated, allowing to identify 124 candidate genes that have a functional role in flower development, stress response, or involvement in important metabolic pathways. Signatures of selection that occurred during olive domestication, were detected and reported. Conclusion: This deep EST&ndash SNP analysis provided important information on the genetic and genomic diversity of the olive complex, opening new opportunities to detect gene polymorphisms with potential functional and evolutionary roles, and to apply them in genomics-assisted breeding, highlighting the importance of olive germplasm conservation. |
| Databáze: | OpenAIRE |
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