Bacterial Quality, Prevalence of Pathogens, and Molecular Characterization of Biofilm-Producing Staphylococcus aureus from Korean Dairy Farm Environments
| Autor: | Sangnam Oh, Hye-Jin Choi, Woongji Lee, Minhye Shin, Sangdon Ryu, Younghoon Kim, Bohyun Yun, Minkyoung Kang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
General Veterinary
biology dairy farm environment Aerobic bacteria Veterinary medicine Bacillus cereus Biofilm Staphylococcus aureus Pathogenic bacteria Raw milk biology.organism_classification medicine.disease_cause biofilm Microbiology QL1-991 Psychrotrophic bacteria SF600-1100 medicine Animal Science and Zoology microbial contamination RNA-seq Zoology foodborne pathogens Bacteria |
| Zdroj: | Animals Volume 11 Issue 5 Animals, Vol 11, Iss 1306, p 1306 (2021) |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani11051306 |
| Popis: | Raw milk acts as a mediator of major foodborne pathogenic bacterial infections. However, the sources of pathogens that contaminate milk are often unclear. This study assessed the prevalence of sanitary quality-indicating bacteria (total aerobic bacteria, psychrotrophic bacteria, coliform, and yeast/molds), including seven foodborne pathogens, in a dairy farm environment and processing plant in Korea. The microbiological analysis showed that a few sites, such as vat bottoms, room floors, drain holes, and niches, showed high microbial loads in most dairy farms. Based on quantitative microbial tests, Bacillus cereus was detected in three farms and Staphylococcus aureus was detected in only one farm. Among them, S. aureus JDFM SA01 isolated from a milk filter showed strong biofilm formation and toxicity to the host Caenorhabditis elegans. Subsequently, RNA-seq was performed to characterize the biofilm formation ability of S. aureus JDFM SA01. In biofilms, the significant upregulation of genes encoding microbial surface components and recognizing adhesive matrix molecules promotes adhesion might explain the increased viability and biomass of biofilms. This study provided insight into the prevalence of pathogenic bacteria and microbial contamination levels across dairy farms. |
| Databáze: | OpenAIRE |
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