Differential Ly49e Expression Pathways in Resting versus TCR-Activated Intraepithelial γδ T Cells
| Autor: | Els Van Ammel, Tessa Kerre, Bart Vandekerckhove, Tina Van Den Broeck, Georges Leclercq, Maarten Delforche, Sylvie Taveirne, Tom Taghon, Jean Plum |
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| Rok vydání: | 2013 |
| Předmět: |
Transcription
Genetic T-Lymphocytes T cell Receptor expression Immunology Mice Transgenic Biology Lymphocyte Activation Epithelium Mice Interleukin 21 medicine Animals Immunology and Allergy Cytotoxic T cell IL-2 receptor Promoter Regions Genetic ZAP70 Lymphotoxin alpha1 beta2 Heterotrimer Receptors Antigen T-Cell gamma-delta Urokinase-Type Plasminogen Activator Cell biology Intestines Killer Cells Natural medicine.anatomical_structure Epidermal Cells Gene Expression Regulation Interleukin 12 CD94/NKG2 Epidermis NK Cell Lectin-Like Receptor Subfamily A Signal Transduction |
| Zdroj: | The Journal of Immunology. 190:1982-1990 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | The Ly49 NK receptor family in mice is composed of several members that recognize MHC class I (MHC-I) or MHC-I–related molecules. We and others have shown before that Ly49E is a unique member, with a different expression pattern on NK cells and being triggered by the non–MHC-I–related protein urokinase plasminogen activator. Among the entire Ly49 receptor family, Ly49E is the only Ly49 member expressed by epidermal-localized γδ T cells and their fetal thymic TCRγδ precursors, and it is the most abundantly expressed member on intestinal intraepithelial γδ T cell lymphocytes. In this study, we provide mechanistic insights into the regulation of Ly49e expression in γδ T cells. First, we demonstrate that TCR-mediated activation of intraepithelial γδ T cells significantly increases Ly49E expression. This results from de novo Ly49E expression and is highly selective, because no other Ly49 family members are induced. TCR-mediated Ly49E induction is a conserved feature of skin- and gut-residing intraepithelial-localized γδ T cell subsets, whereas it is not observed in spleen γδ T cells. By investigating Ly49e promoter activities and lymphotoxin (LT) αβ dependency in resting versus TCR-activated intraepithelial γδ T cells, we reveal two separate regulatory pathways for Ly49E expression, as follows: a LTαβ-dependent pathway leading to basal Ly49E expression in resting cells that is induced by Pro2-mediated Ly49e transcription, and a LTαβ-independent pathway leading to elevated, Pro3-driven Ly49E expression in TCR-stimulated cells. |
| Databáze: | OpenAIRE |
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