Evaluation of 3 analyte-specific reagents for detection of Bordetella pertussis and Bordetella parapertussis in clinical specimens
| Autor: | Ferdaus Hassan, Jeremiah Bell, Rangaraj Selvarangan, Lindsay Hays |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Detection limit Bordetella pertussis Analyte Bacteriological Techniques Bordetella parapertussis biology General Medicine biology.organism_classification Sensitivity and Specificity Confidence interval law.invention Microbiology Bordetella Infectious Diseases Real-time polymerase chain reaction Molecular Diagnostic Techniques law Nasopharynx Humans Reagent Kits Diagnostic Polymerase chain reaction Bordetella Infections |
| Zdroj: | Diagnostic microbiology and infectious disease. 80(3) |
| ISSN: | 1879-0070 |
| Popis: | The performance of 3 analyte-specific reagents (ASRs), Elitech Biosciences, EraGen Biosciences, and Focus Diagnostic, was evaluated for detection of Bordetella pertussis (BP) and Bordetella parapertussis (BPP) in nasopharyngeal swab specimens. A total of 104 frozen, leftover clinical specimens obtained from pediatric patients during 2011-2012 were included in this study. Performance was compared to the Bordetella real-time polymerase chain reaction (PCR) laboratory-developed test (LDT). The positive percent agreement for detection of BP by Elitech was 96% (95% confidence interval [CI]: 85.14-99.30); EraGen and Focus was 98% (95% CI: 87.99-99.89) in comparison to LDT PCR assay. The negative percent agreement of Elitech, EraGen, and Focus in comparison to LDT was 96% (95% CI: 85.14-99.30), 92% (95% CI: 79.89-97.41), and 96% (95% CI: 85.14-99.30), respectively. Limit of detection (LOD) for BP was 0.1 CFU/reaction by both Focus and EraGen and 1.0 CFU/reaction by Elitech. However, LOD for BPP was lower by EraGen (0.1 CFU/reaction) compared to Focus (1.0 CFU/reaction) and Elitech (1.0 CFU/reaction). These results demonstrate that all 3 ASRs tested are comparable and reliable for routine clinical diagnosis of pertussis and parapertussis. |
| Databáze: | OpenAIRE |
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