Inhibition of Low-Density Lipoprotein Oxidation and Up-Regulation of Low-Density Lipoprotein Receptor in HepG2 Cells by Tropical Plant Extracts
| Autor: | Paul D. Roach, Irine Runnie, Mahinda Y. Abeywardena, Mohammed Nizar Salleh, Suhaila Mohamed |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Carcinoma Hepatocellular Antioxidant Thiobarbituric acid medicine.medical_treatment Biology Thiobarbituric Acid Reactive Substances Antioxidants chemistry.chemical_compound Tumor Cells Cultured medicine TBARS Humans Food science Tropical Climate Tea Plant Extracts Liver Neoplasms fungi food and beverages General Chemistry Lipoproteins LDL Kinetics Receptors LDL chemistry Biochemistry Polyphenol Low-density lipoprotein Shoot LDL receptor lipids (amino acids peptides and proteins) Lipid Peroxidation Plants Edible General Agricultural and Biological Sciences Copper Lipoprotein |
| Zdroj: | Journal of Agricultural and Food Chemistry. 50:3693-3697 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf011593f |
| Popis: | Twelve edible plant extracts rich in polyphenols were screened for their potential to inhibit oxidation of low-density lipoprotein (LDL) in vitro and to modulate LDL receptor (LDLr) activity in cultured HepG2 cells. The antioxidant activity (inhibition of LDL oxidation) was determined by measuring the formation of conjugated dienes (lag time) and thiobarbituric acid reagent substances (TBARS). Betel leaf (94%), cashew shoot (63%), Japanese mint (52%), semambu leaf (50%), palm frond (41%), sweet potato shoot, chilli fruit, papaya shoot, roselle calyx, and maman showed significantly increased lag time (>55 min, P < 0.05) and inhibition of TBARS formation (P < 0.05) compared to control. LDLr was significantly up-regulated (P < 0.05) by Japanese mint (67%), semambu (51%), cashew (50%), and noni (49%). Except for noni and betel leaf, most plant extracts studied demonstrated a positive association between antioxidant activity and the ability to up-regulate LDL receptor. Findings suggest that reported protective actions of plant polyphenols on lipoprotein metabolism might be exerted at different biochemical mechanisms. |
| Databáze: | OpenAIRE |
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