Apoptosis in pancreatic β-cells is induced by arsenic and atorvastatin in Wistar rats with diabetes mellitus type 2
| Autor: | Tania Guadalupe Delgado-León, Fernando Vazquez-Alaniz, Eduardo Lozano-Guzmán, Olga Dania López-Guzmán, José M. Salas-Pacheco, Maribel Cervantes-Flores, Norma Urtiz-Estrada, Aurora Martínez-Romero, Angel Antonio Vértiz-Hernández |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Blood Glucose Male medicine.medical_specialty Atorvastatin chemistry.chemical_element 030209 endocrinology & metabolism Apoptosis Biochemistry Rats Inbred WKY Antioxidants Streptozocin Arsenic Diabetes Mellitus Experimental Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Diabetes mellitus Insulin-Secreting Cells medicine Animals Whole blood nutritional and metabolic diseases medicine.disease Streptozotocin Blot 030104 developmental biology Endocrinology chemistry Diabetes Mellitus Type 2 Molecular Medicine Female Glycated hemoglobin Hydroxymethylglutaryl-CoA Reductase Inhibitors medicine.drug |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 46 |
| ISSN: | 1878-3252 |
| Popis: | Diabetes Mellitus type 2 (T2D) is a multifactorial disease. However, it is known that there is an important effect in pancreatic β-cells caused by apoptosis of pro-apoptotic proteins, possibly related to arsenic exposure and atorvastatin treatment.The goal of this study was to evaluate the effects of atorvastatin treatment on apoptosis of pancreatic β-cells in Wistar rats with induced diabetes type 2 exposed to arsenic.T2D in Wistar rats was induced by administration of Streptozotocin. The plasmatic glucose concentrations were measured using the glucose oxidase method, and the concentration of glycated hemoglobin (HbA1c) in whole blood was determined. Exposure to arsenic was measured from urine using atomic absorption with hydride generation, and pro-apoptotic proteins in pancreatic β-cells were observed using the Western blotting technique.Caspase-3 was present in rats that were treated with 10 mg/kg of oral atorvastatin and exposed to 0.01 and 0.025 mg/L of arsenic, but no others proteins were present, such as pro Caspase-8, bcl-2, and Fas. The glycemic levels were 129.2 ± 7.0 mg/dL in the control group and 161.8 ± 14.6 mg/dL and 198.3 ± 18.2 mg/dL (p .05) in the study groups. HbA1c increased from 2.53% to 3.64% (p .05) in the control and study groups.Atorvastatin treatment and arsenic exposure alone are capable of generating apoptosis in pancreatic β-cells of Wistar rats with T2D. Together, all of these factors induce apoptosis in pancreatic cells. |
| Databáze: | OpenAIRE |
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