Knockdown of Circular RNA hsa_circ_PVT1 Inhibited Laryngeal Cancer Progression via Preventing wnt4/β-Catenin Signaling Pathway Activation
| Autor: | Ying Lin, Jia-Li Huang, Mao-Mao Ai, Feng Yu, Guo-Jie Tan, Zi-Rou Zou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Flow cytometry 03 medical and health sciences Cell and Developmental Biology 0302 clinical medicine has_circ_PVT1 medicine Gene silencing Biology (General) Original Research Gene knockdown TUNEL assay medicine.diagnostic_test Cell growth Chemistry Cancer Cell Biology Cell cycle medicine.disease 030104 developmental biology wnt4/β-catenin signal pathway cell proliferation Apoptosis 030220 oncology & carcinogenesis Cancer research laryngeal cancer Developmental Biology miR-21-5p/CBX4 |
| Zdroj: | Frontiers in Cell and Developmental Biology Frontiers in Cell and Developmental Biology, Vol 9 (2021) |
| ISSN: | 2296-634X |
| Popis: | AimTo explore the function and mechanism of circular has_circ_PVT1 on laryngeal cancer (LC).MethodsMicroarray chip was performed to screen the differential expression of circRNA. Western blot and qRT-PCR was employed to detect the protein and mRNA level. CCK-8, clone formation, cell cycle, wound healing, and Transwell assay were performed to detect the cell proliferation, migration, and invasion ability. Luciferase assay and Fish were used to confirm the relationship between circ_PVT1/CBX4 and miR-21-5p. Flow cytometry and TUNEL assay were carried out to assess the apoptosis level.ResultsThe upregulation of circ_PVT1 was found in LC tissues and cells. Silencing of circ_PVT1 inhibited LC progression via targeting miR-21-5p and indirectly controlling CBX4. Wnt4/β-catenin signal pathway was inactivated by inhibiting the expression of circ_PVT1.ConclusionKnockdown of circ_PVT1 prevented LC progression via targeting miR-21-5p/CBX4 by inhibiting wnt4/β-catenin signal pathway, which could provide a novel therapeutic target for LC. |
| Databáze: | OpenAIRE |
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