Profiles of coagulase-positive and -negative staphylococci in retail pork: prevalence, antimicrobial resistance, enterotoxigenicity, and virulence factors
| Autor: | Soo-Jin Yang, Gi Yong Lee |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
040301 veterinary sciences
Tetracycline Physiology pork virulence factors Virulence Enterotoxin Biology Article Microbiology 0403 veterinary science Antibiotic resistance Animal Products lcsh:Zoology medicine Genetics antimicrobial resistance lcsh:QL1-991 staphylococci General Veterinary cons 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences Antimicrobial 040201 dairy & animal science Staphylococcal Food Poisoning Animal Science and Zoology Coagulase staphylococcal enterotoxins (ses) medicine.drug Food Science |
| Zdroj: | Animal Bioscience, Vol 34, Iss 4, Pp 734-742 (2021) Animal Bioscience |
| ISSN: | 2765-0235 2765-0189 |
| DOI: | 10.5713/ab.20.0660 |
| Popis: | Objective: The present study aimed to investigate the occurrence and species of coagulasepositive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS) in retail pork meat samples collected during nationwide monitoring. The staphylococcal isolates were characterized for antimicrobial and zinc chloride resistance and enterotoxigenic potential.Methods: A total of 260 pre-packaged pork meat samples were collected from 35 retail markets in 8 provinces in Korea for isolation of staphylococci. Antimicrobial and zinc chloride resistance phenotypes, and genes associated with the resistance phenotypes were determined on the isolates. Furthermore, the presence and distribution of 19 staphylococcal enterotoxin (SE) genes and enterotoxin-like genes among the pork-associated staphylococci were determined by multiplex polymerase chain reaction-based assays using the specific primer sets.Results: A total of 29 staphylococcal strains (29/260, 11.1%) were isolated from samples of retail pork meat, 24 (83%) of which were CoNS. The four CoNS species identified were S. saprophyticus (n = 16, 55%), S. sciuri (n = 3, 10%), S. warneri (n = 3, 10%), and S. epidermidis (n = 2, 7%). Among the 29 isolates, four methicillin-resistant CoNS (MR-CoNS; three S. sciuri and one S. epidermidis) and one methicillin-resistant CoPS (MR-CoPS; one S. aureus) were identified. In addition, a relatively high level of tetracycline (TET) resistance (52%) was confirmed in CoNS, along with a predominant distribution of tet(K). The most prevalent SEs were sep (45%), and sen (28%), which were carried by 81% of S. saprophyticus.Conclusion: These findings suggest that CoNS, especially S. saprophyticus strains, in raw pork meat could be a potential risk factor for staphylococcal food poisoning (SFP), and therefore, requires further investigation to elucidate the role of SEls in SFP and virulence of the pathogen. Our results also suggest that CoNS from raw pork meat may act as a source for transmission of antimicrobial resistance genes such as staphylococcal cassette chromosome mec and tet(K). |
| Databáze: | OpenAIRE |
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