Phenotypic and genotypic alterations associated with the attenuation of a Theileria annulata vaccine cell line from Turkey
| Autor: | Varda Shkap, Paul Littlebury, Elizabeth Glass, C.G. Duncan Brown, F. Roger Hall, Robert A. Oliver, Eugene Pipano, Rob P.T. Somerville, Rachel Adamson |
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| Rok vydání: | 1998 |
| Předmět: |
Protozoan Vaccines
Genotype Transcription Genetic Population Down-Regulation Virulence Context (language use) Vaccines Attenuated Polymerase Chain Reaction Gene expression Animals RNA Messenger education Regulation of gene expression education.field_of_study Attenuated vaccine General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Metalloendopeptidases Babesia bovis biology.organism_classification Theileria annulata Virology Theileriasis Phenotype Infectious Diseases Gene Expression Regulation Cell culture Molecular Medicine Cattle Polymorphism Restriction Fragment Length |
| Zdroj: | Vaccine. 16:569-575 |
| ISSN: | 0264-410X |
| Popis: | Attenuated vaccines, produced by prolonged in vitro culture of the macroschizont stage of the life-cycle, are the main method of controlling Theileria annulata infections. Little is known about the mechanism(s) of attenuation. Here we present data from a Turkish cell line demonstrating that attenuation is associated with reduced ability to differentiate into microschizonts and a reduction in matrix metalloproteinase activity. We also show that attenuation results in a change in the structure of the parasite population. Using the technique of differential mRNA display, we demonstrate that gene expression profiles differ between non-attenuated and attenuated macroschizont infected leucocytes. One differentially expressed gene is of parasite origin. These data are discussed in the context of a multifactorial model for virulence. |
| Databáze: | OpenAIRE |
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