Phytochemicals inMorinda citrifoliafruit selectively modulate age-associated immunity and antioxidant enzyme activities through ERK pathway in splenic lymphocytes of male F344 rats
| Autor: | Hannah P. Priyanka, Uday P. Pratap, Lalgi Hima, Krithika Anand, Srinivasan ThyagaRajan, Fariya Yasmine |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Aging Antioxidant MAP Kinase Signaling System medicine.medical_treatment Phytochemicals Pharmacology Nitric Oxide Biochemistry Antioxidants Superoxide dismutase Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Lymphocytes Morinda Molecular Biology chemistry.chemical_classification biology Glutathione peroxidase Immunity Cell Biology biology.organism_classification Rats Cytokine Glutathione S-transferase chemistry Catalase Fruit 030220 oncology & carcinogenesis biology.protein Lipid Peroxidation Oxidation-Reduction Spleen 030217 neurology & neurosurgery |
| Zdroj: | Journal of Receptors and Signal Transduction. 36:139-151 |
| ISSN: | 1532-4281 1079-9893 |
| DOI: | 10.3109/10799893.2015.1061001 |
| Popis: | The mechanisms of immunomodulatory effects of Morinda citrifolia (Noni) were examined through intracellular signaling pathways in the splenocytes and their modulation by phytochemicals using bioinformatics tools.Noni fruit juices without seeds (NSL) and with seeds (NWS) were co-incubated in vitro with splenocytes from young, middle-aged and old F344 male rats and proliferation of lymphocytes, cytokine production, antioxidant enzyme activities and intracellular signaling markers were measured.NSL decreased lymphoproliferation in early middle-aged rats, and IL-2 and IFN-γ production in old rats. In contrast, NWS enhanced lymphoproliferation in young and old rats, IL-2 and IFN-γ production in middle-aged and old rats. The activities of antioxidant enzymes were augmented by NWS and NSL in old rats. NWS reversed age-related increase in lipid peroxidation in all age-groups, while NSL increased lipid peroxidation in old rats. NSL increased p-ERK in old rats and decreased p-CREB in young and middle-aged rats. In contrast, NWS decreased p-ERK in all age groups and increased p-CREB in old rats. Both NSL and NWS increased p-Akt expression in middle-aged and old rats. Both NSL and NWS suppressed p-NF-κB expression in middle-aged and old rats. Docking studies demonstrated that Noni phytochemicals, damnacanthal, myricetin and ursolic acid, are potent inhibitors of ERK with binding sites in the catalytic and phosphorylation sites of the molecule.These results suggest that Noni fruit juices with or without seeds modulate cell-mediated immunity and antioxidant enzyme activities based on the phytochemicals that may differentially influence cell signaling and therefore, age-associated immunity. |
| Databáze: | OpenAIRE |
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