Circulating Tumor Cells With Epithelial–to–mesenchymal Transition Phenotypes Associated With Inferior Outcomes in Primary Breast Cancer
| Autor: | Massimo Cristofanilli, Jozef Mardiak, Tatiana Sedlackova, Denisa Manasova, Daniel Pindak, Katarina Kalavska, Gabriel Minarik, Juraj Benca, James M. Reuben, Jurisova Silvia, Michal Mego, Marian Karaba |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Cancer Research Epithelial-Mesenchymal Transition Time Factors Adolescent Slug Breast Neoplasms Peripheral blood mononuclear cell Disease-Free Survival Young Adult 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Humans Medicine Prospective Studies Epithelial–mesenchymal transition Child Transcription factor Aged Aged 80 and over biology business.industry Twist-Related Protein 1 Nuclear Proteins Zinc Finger E-box-Binding Homeobox 1 General Medicine Middle Aged Neoplastic Cells Circulating biology.organism_classification Phenotype Oncology Case-Control Studies Child Preschool 030220 oncology & carcinogenesis embryonic structures Cancer cell Cancer research Female Snail Family Transcription Factors Primary breast cancer business |
| Zdroj: | Anticancer Research. 39:1829-1837 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13290 |
| Popis: | Background/aim Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. Patients and methods This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). Results In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. Conclusion CTCs with an EMT phenotype have a prognostic value in primary breast cancer. |
| Databáze: | OpenAIRE |
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