Screening Arabidopsis mutants in genes useful for phytoremediation
| Autor: | Ana Sevilla-Perea, María C. Romero-Puertas, Luisa M. Sandalio, Liliana B. Pena, María Rodríguez-Serrano, María Sanz-Fernández, M. Dolores Mingorance |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Chromium Health Toxicology and Mutagenesis ATPase Mutant Arabidopsis Chromosomal translocation 010501 environmental sciences 01 natural sciences Soil Pollutants Waste Management and Disposal Genetics Pollution Phenotype Biodegradation Environmental Heavy metals Screening Chaperons CIENCIAS NATURALES Y EXACTAS Signal Transduction Cadmium DNA Bacterial Environmental Engineering DNA Plant Otras Ciencias Biológicas Biology Genes Plant Mining Ciencias Biológicas Metals Heavy Botany Environmental Chemistry Gene Ecosystem 0105 earth and related environmental sciences Screeninga Arabidopsis Proteins Wild type Biological Transport biology.organism_classification Phytoremediation Spain Oxidative stress Mutation biology.protein Chrome Copper 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Emissions of heavy metals have risen over the past 200 years and significantly exceed those from natural sources. Phytoremediation strategies may be able to recover soil productivity in self-sustaining ecosystems; however, our knowledge of the molecular mechanisms involved in plant heavy-metal perception and signalling is scarce. The aim of this study was to assemble a “molecular tool box” of genes useful for phytoremediation. To identify mutants with different heavy-metal-tolerance, we first selected a medium from mixtures containing three metals based on their presence in two Spanish mining areas and then screened about 7000 lines of Arabidopsis T-DNA mutants and found 74 lines more resistant and 56 more susceptible than the wild type (WT). Classification of the genes showed that they were mainly linked to transport, protein modification and signalling, with RNA metabolism being the most representative category in the resistant phenotypes and protein metabolism in the sensitive ones. We have characterized one resistant mutant, Athpp9 and one sensitive, Atala4. These mutants showed differences in growth and metal translocation. Additionally, we found that these mutants keep their phenotype in amended former soils, suggesting that these genes may be useful for phytoremediation and the recovery of contaminated soils. This study was supported by the Fundación Ramón Areces(http://www.fundacionareces.es) Spain, by ERDF co-financed grant BIO2015-67657-P from MICINN and the Junta de Andalucía (BIO-337). MRS is grateful to the CSIC and European Social Fund (ESF) fora JAE-DOC contract. The English text was corrected by Ms. AngelaTate. |
| Databáze: | OpenAIRE |
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