beta-Carotene stimulates chemotaxis of human endothelial progenitor cells
| Autor: | Jadwiga Hartwich, Anna Polus, Joanna Skrzeczyńska, Magdalena Mikolajczyk, Juliusz Pryjma, Aldona Dembinska-Kiec, Beata Kieć-Wilk, Joanna Grzybowska |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
$\beta-carotene$
Angiogenesis Cellular differentiation Clinical Biochemistry Biological Transport Active Gene Expression Neovascularization Physiologic Biology Endothelial progenitor cell endothelial progenitor cell arachidonic acid Humans Progenitor cell chemotaxis Cells Cultured Cell Proliferation Matrigel Arachidonic Acid Cell growth Chemotaxis Stem Cells Biochemistry (medical) Cell Differentiation General Medicine beta Carotene Cell biology Endothelial stem cell Immunology Endothelium Vascular Stem cell homing |
| Zdroj: | Clinical chemistry and laboratory medicine. 43(5) |
| ISSN: | 1434-6621 |
| Popis: | Angiogenesis is a crucial process in tissue remodeling during growth, both in the embryo and the adult. In our study we concentrated on the direct effect of β-carotene on human umbilical cord originating from endothelial progenitor cells (EPCs). β-Carotene uptake by EPCs was measured using a HPLC method. The determination of cell surface antigens was performed by flow cytometry. The effect on cell proliferation was estimated by measuring bromo-deoxyuridine incorporation. The influence on the formation of a tubular-like structure was investigated in a 3D assay in matrigel. Quantitative gene expression was estimated using real-time PCR. We demonstrated that β-carotene in the physiological range of concentrations found in human blood is a potent activator of EPC chemotaxis, which is accompanied by a change in the expression of genes mediating cell adhesion and homing, but does not activate the final markers of endothelial differentiation. This study points to the prochemotactic and homing activity of β-carotene in undifferentiated endothelial cell progenitors for the first time, which may suggest a potential role of this carotenoid in progenitor cell therapy aimed at angiogenesis and tissue repair. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|