Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers
| Autor: | Christian J. Isaac, Virtudes Moreno, Syma Khalid, Michael J. Hannon, Alison Rodger, Einar Sletten, Isabelle Meistermann, Erlend Moldrheim, P. Mark Rodger, Jemma C. Peberdy, María J. Prieto |
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| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular Circular dichroism Stereochemistry Iron Supramolecular chemistry Plasma protein binding Ligands Microscopy Atomic Force chemistry.chemical_compound Molecule Animals Multidisciplinary Circular Dichroism Temperature DNA Hydrogen-Ion Concentration Binding constant Crystallography chemistry Models Chemical Metals Spectrophotometry Intramolecular force Physical Sciences Nucleic Acid Conformation Cattle Enantiomer Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 99(8) |
| ISSN: | 0027-8424 |
| Popis: | We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10 7 M −1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer. |
| Databáze: | OpenAIRE |
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