Effects of a phytosterol mixture on male fish plasma lipoprotein fractions and testis P450scc activity
| Autor: | Christine Gilman, W. Carl Breckenridge, Frederic D.L. Leusch, Deborah L. MacLatchy |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Trout Lipoproteins Industrial Waste chemistry.chemical_compound Random Allocation Endocrinology Internal medicine Goldfish Testis medicine Animals Cholesterol Side-Chain Cleavage Enzyme Triglycerides biology Triglyceride Cholesterol Phytosterol Cholesterol side-chain cleavage enzyme Reproduction Phytosterols Environmental exposure Cholesterol LDL Environmental Exposure biology.organism_classification Sitosterols Mitochondria chemistry Sex steroid Pregnenolone lipids (amino acids peptides and proteins) Animal Science and Zoology Water Pollutants Chemical medicine.drug |
| Zdroj: | General and comparative endocrinology. 130(2) |
| ISSN: | 0016-6480 |
| Popis: | Plant sterols (phytosterols) have been identified as one potential source of reproductive effects in fish living downstream of pulp mills. beta-Sitosterol, the predominant plant sterol in pulp mill effluent, has previously been shown to decrease plasma sex steroid and cholesterol levels and in vitro gonadal steroid production in fish. In this study, male brook trout (Salvelinus fontinalis) and goldfish (Carassius auratus) were exposed to a phytosterol mixture (72% beta-sitosterol) via Silastic intraperitoneal implants to help elucidate the mechanisms of action of phytosterols on steroid depression. As cholesterol is exogenously supplied for gonadal steroidogenesis, changes in plasma cholesterol fractions were examined. In male brook trout, low-density lipoprotein cholesterol and triglyceride levels decreased significantly, 43 and 50%, respectively, in phytosterol-treated fish. It is improbable, however, that these decreases are linked to depressed gonadal steroidogenesis in fish. The activity of P450scc, which converts cholesterol to pregnenolone (the first step in the steroidogenic pathway), was not affected in testis mitochondria isolated from brook trout or goldfish. Further investigation of the mechanisms of action of phytosterols is required. |
| Databáze: | OpenAIRE |
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