Scaffolds from Self-Assembling Tetrapeptides Support 3D Spreading, Osteogenic Differentiation, and Angiogenesis of Mesenchymal Stem Cells
Autor: | Salwa Alshehri, Charlotte A. E. Hauser, Hepi Hari Susapto |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Polymers and Plastics
Angiogenesis Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Article Biomaterials Extracellular matrix 3D cell culture Tissue engineering Osteogenesis Materials Chemistry Humans Bone regeneration Tetrapeptide Tissue Engineering Tissue Scaffolds Chemistry Mesenchymal stem cell technology industry and agriculture Cell Differentiation Hydrogels Mesenchymal Stem Cells 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology Self-healing hydrogels 0210 nano-technology |
Zdroj: | Biomacromolecules |
ISSN: | 1526-4602 1525-7797 |
Popis: | The apparent rise of bone disorders demands advanced treatment protocols involving tissue engineering. Here, we describe self-assembling tetrapeptide scaffolds for the growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs). The rationally designed peptides are synthetic amphiphilic self-assembling peptides composed of four amino acids that are nontoxic. These tetrapeptides can quickly solidify to nanofibrous hydrogels that resemble the extracellular matrix and provide a three-dimensional (3D) environment for cells with suitable mechanical properties. Furthermore, we can easily tune the stiffness of these peptide hydrogels by just increasing the peptide concentration, thus providing a wide range of peptide hydrogels with different stiffnesses for 3D cell culture applications. Since successful bone regeneration requires both osteogenesis and vascularization, our scaffold was found to be able to promote angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro. The results presented suggest that ultrashort peptide hydrogels are promising candidates for applications in bone tissue engineering. |
Databáze: | OpenAIRE |
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