Discovery of bioactive nitrated lipids and nitro-lipid-protein adducts using mass spectrometry-based approaches
| Autor: | Javier-Fernando Montero-Bullon, M. Rosário M. Domingues, Tânia Melo, Pedro Domingues |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Lipoproteins Clinical Biochemistry Mass spectrometry Biochemistry Mass Spectrometry Adduct 03 medical and health sciences Residue (chemistry) 0302 clinical medicine Protein structure Lipoxidation in pathophysiology and its assessment by high precision methodology lcsh:QH301-705.5 Histidine lcsh:R5-920 Nitrates Molecular Structure Chemistry Fatty Acids Organic Chemistry Nitro Compounds Subcellular localization Lipids 3. Good health 030104 developmental biology lcsh:Biology (General) Signal transduction lcsh:Medicine (General) Oxidation-Reduction Protein Processing Post-Translational Biomarkers 030217 neurology & neurosurgery Signal Transduction Cysteine |
| Zdroj: | Redox Biology Redox Biology, Vol 23, Iss, Pp-(2019) |
| ISSN: | 2213-2317 |
| DOI: | 10.1016/j.redox.2019.101106 |
| Popis: | Nitro-fatty acids (NO2-FA) undergo reversible Michael adduction reactions with cysteine and histidine residues leading to the post-translational modification (PTM) of proteins. This electrophilic character of NO2-FA is strictly related to their biological roles. The NO2-FA-induced PTM of signaling proteins can lead to modifications in protein structure, function, and subcellular localization. The nitro lipid-protein adducts trigger a series of downstream signaling events that culminates with anti-inflammatory, anti-hypertensive, and cytoprotective effects mediated by NO2-FA. These lipoxidation adducts have been detected and characterized both in model systems and in biological samples by using mass spectrometry (MS)-based approaches. These MS approaches allow to unequivocally identify the adduct together with the targeted residue of modification. The identification of the modified proteins allows inferring on the possible impact of the NO2-FA-induced modification. This review will focus on MS-based approaches as valuable tools to identify NO2-FA-protein adducts and to unveil the biological effect of this lipoxidation adducts. Highlights • Nitro-fatty acids (NO2-FA) are endogenous bioactive lipids. • NO2-FA form reversible Michael adducts with proteins leading to PTMs. • Adduction of NO2-FA with proteins culminates to anti-inflammatory, anti-hypertensive, and cytoprotective effects. • Mass spectrometry (MS)-based approaches allows to identify NO2-FA-protein adducts and to unveil their biological effects. |
| Databáze: | OpenAIRE |
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