Cell migration towards CXCL12 in leukemic cells compared to breast cancer cells
| Autor: | Will Maxwell, Anja Mueller, Jossie Kudatsih, Poh Hui Goh, Sithembile Ncube, Shirley C. Mills, Renu Gurung |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
STAT3 Transcription Factor Cell type Morpholines Cell Breast Neoplasms Protein Kinase C-epsilon Biology Jurkat cells Proto-Oncogene Proteins p21(ras) 03 medical and health sciences Jurkat Cells medicine Humans PI3K/AKT/mTOR pathway Leukemia Chemotaxis Cell migration Cell Biology Janus Kinase 2 Chemokine CXCL12 Cell biology 030104 developmental biology medicine.anatomical_structure Cell culture Chromones Cancer cell MCF-7 Cells Female |
| Zdroj: | Cellular signalling. 28(4) |
| ISSN: | 1873-3913 |
| Popis: | Chemotaxis or directed cell migration is mediated by signalling events initiated by binding of chemokines to their cognate receptors and the activation of a complex signalling cascade. The molecular signalling pathways involved in cell migration are important to understand cancer cell metastasis. Therefore, we investigated the molecular mechanisms of CXCL12 induced cell migration and the importance of different signalling cascades that become activated by CXCR4 in leukemic cells versus breast cancer cells. We identified Src kinase as being essential for cell migration in both cancer types, with strong involvement of the Raf/MEK/ERK1/2 pathway. We did not detect any involvement of Ras or JAK2/STAT3 in CXCL12 induced migration in Jurkat cells. Preventing PKC activation with inhibitors does not affect migration in Jurkat cells at all, unlike in the adherent breast cancer cell line MCF-7 cells. However, in both cell lines, knock down of PKCα prevents migration towards CXCL12, whereas the expression of PKCζ is less crucial for migration. PI3K activation is essential in both cell types, however LY294002 usage in MCF-7 cells does not block migration significantly. These results highlight the importance of verifying specific signalling pathways in different cell settings and with different approaches. |
| Databáze: | OpenAIRE |
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