Induction of oxidative stress by chlorothalonil in the estuarine polychaete Laeonereis acuta
| Autor: | Juliano da Silva Barreto, Márcio Alberto Geihs, Filipe Guilherme Andrade de Godoi, Fiamma Eugênia Lemos Abreu, Juliana Zomer Sandrini, Gilberto Fillmann, Carlos Eduardo da Rosa, Fabio de Melo Tarouco |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis 010501 environmental sciences Aquatic Science medicine.disease_cause 01 natural sciences Antioxidants Lipid peroxidation Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Nitriles medicine Animals Cholinesterases Lactic Acid Glutathione Transferase 0105 earth and related environmental sciences Glutathione Peroxidase Chlorothalonil biology Glycogen Superoxide Dismutase Polychaeta Glutathione Catalase Acetylcholinesterase Fungicides Industrial Peroxides Oxidative Stress 030104 developmental biology chemistry Biochemistry Spectrophotometry biology.protein Lipid Peroxidation Water Pollutants Chemical Oxidative stress |
| Zdroj: | Aquatic Toxicology. 196:1-8 |
| ISSN: | 0166-445X |
| DOI: | 10.1016/j.aquatox.2017.12.004 |
| Popis: | Chlorothalonil is an active biocide applied in antifouling paints, and also used as fungicide in agricultural activities with the purpose to protect plants from foliar and seed diseases. Thus, the aim of this study was to evaluate the effects of chlorothalonil exposure on biochemical biomarkers of oxidative metabolism as well as on cholinesterases in the estuarine polychaete Laeonereis acuta. Animals were exposed for 24 and 96 h to the following nominal concentrations of chlorothalonil: 0.1, 10.0 and 100.0 μg/L. The antioxidant capacity against peroxyl radicals (ACAP) and the activity of the enzymes catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutamate cysteine ligase (GCL), acetylcholinesterase (AChE) and propionylcholinesterase (PChE) were evaluated in whole-body tissue. In addition, the levels of reduced glutathione (GSH), lipid peroxidation (LPO), glycogen and lactate levels were also analyzed. A reduction in ACAP levels was observed in animals exposed to the higher chlorothalonil concentration, concomitantly with an induction of GST activity as well as diminution in GSH content in these animals. This disturbance in the redox state of animal tissues leads to an oxidative stress situation, resulting in an induction in LPO levels. It was also demonstrated that chlorothalonil exposure causes alteration in AChE activity, possibly related to damage to membrane lipids. These results demonstrated that chlorothalonil possesses harmful effects to estuarine animals and its use as antifouling biocide has to be carefully reconsidered in risk analysis studies. |
| Databáze: | OpenAIRE |
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