Structure of Trihexosylceramide Biosynthesized in Vitro
| Autor: | Pierre Stoffyn, George Hauser, Anne Stoffyn |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Chemical Phenomena
Stereochemistry Oligosaccharides Lactose Biology Ceramides Tritium Methylation Biochemistry Lactosylceramide Glycolipid Cerebrosides Isomerism Bone Marrow Microsomes Animals Trihexosylceramide music Molecular Biology Galactosyltransferase Carbon Isotopes music.instrument Galactose Cell Biology Uridine Diphosphate Sugars In vitro Galactosidases Rats Chemistry Hexosyltransferases Microsome Autoradiography Rat Spleen Chromatography Thin Layer Glycolipids Spleen |
| Zdroj: | Journal of Biological Chemistry. 248:1920-1923 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(19)44167-7 |
| Popis: | Trihexosylceramide biosynthesized in vitro from lactosylceramide and UDP-galactose in the presence of a galactosyltransferase from rat spleen microsomes or bone marrow was shown by methylation to have the structure galactopyranosyl-α-(1 → 3)-galactopyranosyl-β-(1 → 4)-glucopyranosyl-β-(1 → 1)-ceramide. It is thus different from trihexosylceramide isolated from several natural sources in which the two galactoses are linked 1 → 4. This emphasizes the need for establishing by unequivocal means the structure of glycolipids biosynthesized in vitro. The method described permits this to be done on the very small amounts of material usually available from biosynthetic experiments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|