Microdialysis for detection of renal ischemia after experimental renal transplantation
| Autor: | Anna Krarup Keller, L. Henning Olsen, Kristian Ravlo, Lars Bjørn Stolle, Troels Munch Jørgensen, Torben Kaer Nielsen |
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| Rok vydání: | 2008 |
| Předmět: |
Nephrology
medicine.medical_specialty Pathology Swine Urology Renal cortex Microdialysis Kidney Renal Artery Renal capsule Ischemia medicine.artery Internal medicine medicine Animals Renal artery Renal ischemia business.industry Kidney metabolism medicine.disease Kidney Transplantation medicine.anatomical_structure Female business Kidney disease |
| Zdroj: | Keller, A K, Ravlo, K, Nielsen, T K, Olsen, L H, Jørgensen, T M & Stolle, L B 2009, ' Microdialysis for Detection of Renal Ischemia After Experimental Renal Transplantation ', Journal of Urology . https://doi.org/10.1016/j.juro.2009.03.015 |
| ISSN: | 1527-3792 |
| DOI: | 10.1016/j.juro.2009.03.015 |
| Popis: | Udgivelsesdato: 2009-Aug-18 PURPOSE: We designed an experimental renal transplantation model and evaluated microdialysis as a detector of induced postoperative ischemia, a feared complication that when caused by vascular thrombosis most often causes renal graft loss. MATERIALS AND METHODS: Two microdialysis catheters were placed in the left kidney in 16 pigs, including 1 superficially in the renal cortex and 1 fixed on the renal capsule. Two-hour baseline measurements were made at steady state, after which the kidney was removed and subjected to warm and cold ischemia. It was subsequently re-anastomosed end to end in situ and reperfused for 5 hours. Pigs were then randomized into a total renal artery occlusion and a control group. RESULTS: At baseline there were no changes in local metabolites (glucose, glutamate, glycerol and lactate) and no significant difference between the groups. Glycerol increased 4-fold in each group during cold ischemia but there were no pivotal alterations in other metabolites. After kidney reperfusion glycerol decreased and all metabolites were in steady state after 1 hour. At 30 minutes after postoperative ischemia was introduced there were significant increases in all kidneys in ischemia vs steady state reperfusion levels of cortical lactate, glutamate, glycerol and the lactate-to-glucose ratio (each rank sum test p |
| Databáze: | OpenAIRE |
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