Orexin A injection into the ventral medial prefrontal cortex induces antidepressant-like effects: Possible involvement of local Orexin-1 and Trk receptors
| Autor: | Samia R. L. Joca, Ariandra G. Sartim, L.A. Stanquini |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Central nervous system Prefrontal Cortex Tropomyosin receptor kinase B Motor Activity 03 medical and health sciences Behavioral Neuroscience Orexin-A 0302 clinical medicine Orexin Receptors Internal medicine medicine Animals Receptor trkB Rats Wistar Receptor Prefrontal cortex 030304 developmental biology 0303 health sciences Orexins COMPORTAMENTO Chemistry Depression Brain-Derived Neurotrophic Factor Antagonist Antidepressive Agents Orexin Rats Endocrinology medicine.anatomical_structure Trk receptor 030217 neurology & neurosurgery Stress Psychological Signal Transduction |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1872-7549 |
| Popis: | Rationale Reduced levels of orexin-A (OXA) in the central nervous system (CNS) have been associated with the pathophysiology of depression and its exogenous administration promotes antidepressant-like effect. The mechanisms associated with these effects are, however, not yet known. Herein, we investigated the hypothesis that OXA effects could be associated with orexin 1 receptor (OX1R) and tyrosine receptor kinase B (TrkB) activation, in the ventromedial prefrontal cortex (vmPFC), a brain region that is central to depression neurobiology. Objectives 1. To Investigate the effects induced by OXA administration into the vmPFC; 2. Evaluate the participation of OX1R and TrkB in behavioral responses induced by OXA. Methods Male Wistar rats received intra-vmPFC injections of OXA (10, 50 and 100 pmol/0.2 μL) and were exposed to the forced swimming test (FST) or the open field test (OFT). Independent groups received an intra-vmPFC injection of SB334867 (OX1R antagonist, 10 nmol/0.2 μL) or K252a (non-selective Trk antagonist, 10 pmol/0.2 μL), before local injection of OXA, and were exposed to the same tests. Results OXA injection (100 pmol/0.2 μL) into the vmPFC induced antidepressant-like effect, which was prevented by SB334867 and K252a pretreatments. Conclusion OXA signaling in the vmPFC induces antidepressant-like effect in the FST which is dependent on OX1R and Trk receptors. |
| Databáze: | OpenAIRE |
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