Microbiota composition and inflammatory immune responses upon peroral application of the commercial competitive exclusion product Aviguard® to microbiota-depleted wildtype mice
| Autor: | Nina Biesemeier, Stefan Bereswill, Sigri Kløve, Dennis Weschka, Markus M. Heimesaat, Soraya Mousavi, Claudia Genger |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
host-pathogen-interaction medicine.drug_class 030106 microbiology Antibiotics Colonisation resistance Gut flora probiotic formulations Microbiology law.invention 03 medical and health sciences Probiotic enteropathogenic infection Immune system law colonization resistance medicine Aviguard® B cell Innate immune system competitive exclusion product murine gut microbiota biology inflammatory immune responses biology.organism_classification Original Research Paper 030104 developmental biology medicine.anatomical_structure Apoptosis |
| Zdroj: | European Journal of Microbiology & Immunology |
| ISSN: | 2062-509X |
| Popis: | Non-antibiotic feed additives including competitive exclusion products have been shown effective in reducing pathogen loads including multi-drug resistant strains from the vertebrate gut. In the present study we surveyed the intestinal bacterial colonization properties, potential macroscopic and microscopic inflammatory sequelae and immune responses upon peroral application of the commercial competitive exclusion product Aviguard® to wildtype mice in which the gut microbiota had been depleted by antibiotic pre-treatment. Until four weeks following Aviguard® challenge, bacterial strains abundant in the probiotic suspension stably established within the murine intestines. Aviguard® application did neither induce any clinical signs nor gross macroscopic intestinal inflammatory sequelae, which also held true when assessing apoptotic and proliferative cell responses in colonic epithelia until day 28 post-challenge. Whereas numbers of colonic innate immune cell subsets such as macrophages and monocytes remained unaffected, peroral Aviguard® application to microbiota depleted mice was accompanied by decreases in colonic mucosal counts of adaptive immune cells such as T and B lymphocytes. In conclusion, peroral Aviguard® application results i.) in effective intestinal colonization within microbiota depleted mice, ii.) neither in macroscopic nor in microscopic inflammatory sequelae and iii.) in lower colonic mucosal T and B cell responses. |
| Databáze: | OpenAIRE |
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