X chromosome and autosomal recombination are differentially sensitive to disruptions in SC maintenance
| Autor: | Katherine Kretovich Billmyre, G. Matthew Heenan, R. Scott Hawley, Jay R. Unruh, Zulin Yu, Satomi Takeo, Emily R. Wesley, Cori K. Cahoon |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Multidisciplinary Autosome biology Transverse filament Biological Sciences biology.organism_classification Cell biology 03 medical and health sciences Synaptonemal complex 0302 clinical medicine Meiosis Drosophila melanogaster Homologous recombination Gene 030217 neurology & neurosurgery X chromosome Recombination 030304 developmental biology |
| Popis: | The synaptonemal complex (SC) is a conserved meiotic structure that regulates the repair of double-strand breaks (DSBs) into crossovers or gene conversions. The removal of any central-region SC component, such as the Drosophila melanogaster transverse filament protein C(3)G, causes a complete loss of SC structure and crossovers. To better understand the role of the SC in meiosis, we used CRISPR/Cas9 to construct 3 in-frame deletions within the predicted coiled-coil region of the C(3)G protein. Since these 3 deletion mutations disrupt SC maintenance at different times during pachytene and exhibit distinct defects in key meiotic processes, they allow us to define the stages of pachytene when the SC is necessary for homolog pairing and recombination during pachytene. Our studies demonstrate that the X chromosome and the autosomes display substantially different defects in pairing and recombination when SC structure is disrupted, suggesting that the X chromosome is potentially regulated differently from the autosomes. |
| Databáze: | OpenAIRE |
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