Simultaneous determination of triclabendazole and its metabolites in bovine and goat tissues by liquid chromatography-tandem mass spectrometry
| Autor: | Wenli Zheng, Minqi Qiu, Lifang Zhang, Feiqun Xue, Chengyuan Cai |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Electrospray ionization
Clinical Biochemistry Mass spectrometry Kidney Biochemistry High-performance liquid chromatography Analytical Chemistry Liquid chromatography–mass spectrometry Tandem Mass Spectrometry medicine Animals Triclabendazole Detection limit Anthelmintics Chromatography Chemistry Goats Muscles Selected reaction monitoring Kidney metabolism Cell Biology General Medicine Liver Benzimidazoles Cattle medicine.drug Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 878(30) |
| ISSN: | 1873-376X |
| Popis: | A sensitive liquid chromatography-tandem mass spectrometry method for the simultaneous determination of triclabendazole, its main metabolites (triclabendazole sulphone and triclabendazole sulphoxide) and a marker residue (ketotriclabendazole) in bovine and goat muscle, liver, and kidney samples is developed and validated. Analyte extraction from samples is effectively performed using liquid-liquid extraction by acetonitrile. Chromatographic separation is performed on a C₁₈ reversed-phase column with gradient elution. The analytes are detected by tandem quadrupole mass spectrometry after positive electrospray ionization by multiple reaction monitoring. The limits of detection for analytes are found to be 0.25-2.5 μg/kg in muscle tissues and 1-10 μg/kg in liver and kidney tissues, respectively. The recoveries of edible bovine and goat tissues range from 84.9% to 109.5% when spiked at different levels with analytes, with relative standard deviations generally below 12.8%. |
| Databáze: | OpenAIRE |
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