Comparison of igg subclasses and complement binding activity of autoantibodies from patients with bullous pemphigoid and pemphigus
| Autor: | Yue-Yun Lee, Walter S. Brooks, Jau-Shyong Deng, Edward Abell |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty medicine.drug_class Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Monoclonal antibody Subclass Antibody Specificity immune system diseases Pemphigoid Bullous medicine Humans Immunology and Allergy Longitudinal Studies skin and connective tissue diseases Complement Activation Autoantibodies Skin Skin Diseases Vesiculobullous integumentary system biology business.industry Biochemistry (medical) Public Health Environmental and Occupational Health Autoantibody Complement C3 Hematology Igg subclasses medicine.disease Dermatology eye diseases In vitro Medical Laboratory Technology Pemphigus Immunoglobulin G Immunology biology.protein Bullous pemphigoid Antibody business |
| Zdroj: | Journal of Clinical Laboratory Analysis. 3:307-311 |
| ISSN: | 1098-2825 0887-8013 |
| Popis: | Bullous pemphigoid and pemphigus are autoimmune disorders of skin of unknown etiology and are characterized by the presence of immunoreactants in the skin and circulating autoantibodies to skin components. The distribution of IgG subclass antibodies to intercellular substance (ICS) of pemphigus and basement membrane zone substance (BMZ) of bullous pemphigoid was analyzed by using monoclonal antibodies to human IgG subclasses. IgG4 type anti-BMZ antibody was found in the majority of patients with bullous pemphigoid (88% in skin and 96% in serum). One third to one half of bullous pemphigoid patients had IgG1 and IgG2 anti-BMZ antibodies. The majority of bullous pemphigoid skin (92%) had complement in skin, however only one third of their sera had complement binding activity in vitro. IgG1 anti-ICS antibody was the predominant one in patients with pemphigus (86% in skin and 80% in circulation). IgG4 anti-ICS antibody was seen in two thirds of specimens from pemphigus patients. IgG3 subclass antibody was more frequently seen in pemphigus than in bullous pemphigoid patients. Two-thirds of pemphigus sera were capable of activating complement in vitro. The complement binding activity was directly associated with IgG1 and/or IgG3 subclass antibodies. The possible mechanisms for the restricted IgG4 subclass antibodies in bullous pemphigoid and pemphigus are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|