Collateral Damage: What Effect Does Anti-CD4 and Anti-CD8α Antibody-Mediated Depletion Have on Leukocyte Populations?
| Autor: | So Ri Jung, Tamara Suprunenko, Markus J. Hofer, Thomas M. Ashhurst, Nicholas J. C. King |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
T cell CD8 Antigens T-Lymphocytes Immunology Cell Clone (cell biology) Gating Flow cytometry 03 medical and health sciences Mice 0302 clinical medicine medicine Immunology and Allergy Animals Humans Cells Cultured medicine.diagnostic_test biology Chemistry Antibody-Dependent Cell Cytotoxicity Antibodies Monoclonal Dendritic cell Flow Cytometry Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Virus Diseases CD4 Antigens Collateral damage biology.protein Leukocytes Mononuclear Female Antibody 030215 immunology |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 201(7) |
| ISSN: | 1550-6606 |
| Popis: | Anti-CD4 or anti-CD8α Ab–mediated depletion strategies are widely used to determine the role of T cell subsets. However, surface expression of CD4 and CD8α is not limited to T cells and occurs on other leukocyte populations as well. Using both unbiased t-distributed stochastic neighbor embedding of flow cytometry data and conventional gating strategies, we assessed the impact of anti-CD4 and anti-CD8α Ab–mediated depletion on non–T cell populations in mice. Our results show that anti-CD4 and anti-CD8α Ab injections not only resulted in depletion of T cells but also led to depletion of specific dendritic cell subsets in a dose-dependent manner. Importantly, the extent of this effect varied between mock- and virus-infected mice. We also demonstrate the importance of using a second, noncompeting Ab (clone CT-CD8α) to detect CD8α+ cells following depletion with anti-CD8α Ab clone 2.43. Our study provides a necessary caution to carefully consider the effects on nontarget cells when using Ab injections for leukocyte depletion in all experimental conditions. |
| Databáze: | OpenAIRE |
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