In-Nucleus Hi-C in Drosophila Cells
| Autor: | Rodrigo G. Arzate-Mejía, Ayerim Esquivel-López, Mayra Furlan-Magaril, Rosario Pérez-Molina |
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| Rok vydání: | 2021 |
| Předmět: |
Cell Nucleus
General Immunology and Microbiology biology General Chemical Engineering General Neuroscience Genomics Computational biology biology.organism_classification Genome Chromatin General Biochemistry Genetics and Molecular Biology Drosophila melanogaster medicine.anatomical_structure Gene expression medicine Animals CRISPR Drosophila Drosophila (subgenus) Gene Nucleus Function (biology) |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | The genome is organized into topologically associating domains (TADs) delimited by boundaries that isolate interactions between domains. In Drosophila, the mechanisms underlying TAD formation and boundaries are still under investigation. The application of the in-nucleus Hi-C method described here helped to dissect the function of architectural protein (AP)-binding sites at TAD boundaries isolating the Notch gene. Genetic modification of domain boundaries that cause loss of APs results in TAD fusion, transcriptional defects, and long-range topological alterations. These results provided evidence demonstrating the contribution of genetic elements to domain boundary formation and gene expression control in Drosophila. Here, the in-nucleus Hi-C method has been described in detail, which provides important checkpoints to assess the quality of the experiment along with the protocol. Also shown are the required numbers of sequencing reads and valid Hi-C pairs to analyze genomic interactions at different genomic scales. CRISPR/Cas9-mediated genetic editing of regulatory elements and high-resolution profiling of genomic interactions using this in-nucleus Hi-C protocol could be a powerful combination for the investigation of the structural function of genetic elements. |
| Databáze: | OpenAIRE |
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