Heterogeneous Responses to Antioxidants in Noradrenergic Neurons of the Locus Coeruleus Indicate Differing Susceptibility to Free Radical Content
| Autor: | Fernanda S. Gravina, Rebecca Lim, Dirk F. van Helden, Alan M. Brichta, Ramatis B. de Oliveira, Robert J. Callister |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adrenergic Neurons
Aging Carbonyl Cyanide m-Chlorophenyl Hydrazone Free Radicals Article Subject Action Potentials Mitochondrion Pharmacology In Vitro Techniques Biochemistry Antioxidants chemistry.chemical_compound Mice Cytosol Animals Chromans lcsh:QH573-671 Membrane potential Membrane Potential Mitochondrial Chemistry lcsh:Cytology Depolarization Cell Biology General Medicine Hyperpolarization (biology) Mitochondria Electrophysiology Dithiothreitol nervous system Locus coeruleus Calcium Locus Coeruleus Trolox Protons Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2012 (2012) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0900 |
| DOI: | 10.1155/2012/820285 |
| Popis: | The present study investigated the effects of the antioxidants trolox and dithiothreitol (DTT) on mouse Locus coeruleus (LC) neurons. Electrophysiological measurement of action potential discharge and whole cell current responses in the presence of each antioxidant suggested that there are three neuronal subpopulations within the LC. In current clamp experiments, most neurons (55%; 6/11) did not respond to the antioxidants. The remaining neurons exhibited either hyperpolarization and decreased firing rate (27%; 3/11) or depolarization and increased firing rate (18%; 2/11). Calcium and JC-1 imaging demonstrated that these effects did not change intracellular Ca2+concentration but may influence mitochondrial function as both antioxidant treatments modulated mitochondrial membrane potential. These suggest that the antioxidant-sensitive subpopulations of LC neurons may be more susceptible to oxidative stress (e.g., due to ATP depletion and/or overactivation of Ca2+-dependent pathways). Indeed it may be that this subpopulation of LC neurons is preferentially destroyed in neurological pathologies such as Parkinson’s disease. If this is the case, there may be a protective role for antioxidant therapies. |
| Databáze: | OpenAIRE |
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