MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
| Autor: | Michał Wojciech Szcześniak, Agnieszka Piasecka, Krzysztof Sobczak, Michał Sekrecki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cell biology
Molecular biology Science Biology Muscle Development Article Deep sequencing Myoblasts Transcription (biology) Developmental biology microRNA RNA Precursors medicine Humans Myocyte MEF2C RNA-Seq Muscle Skeletal Uridine Transcription factor Cells Cultured Muscle Cells Gene knockdown Multidisciplinary MEF2 Transcription Factors Skeletal muscle Cell Differentiation Up-Regulation DNA-Binding Proteins MicroRNAs medicine.anatomical_structure Gene Knockdown Techniques Medicine Transcriptome |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Myocyte enhancer factor 2C (MEF2C) is a transcription factor that regulates heart and skeletal muscle differentiation and growth. Several protein-encoding genes were identified as targets of this factor; however, little is known about its contribution to the microtranscriptome composition and dynamics in myogenic programs. In this report, we aimed to address this question. Deep sequencing of small RNAs of human muscle cells revealed a set of microRNAs (miRNAs), including several muscle-specific miRNAs, that are sensitive to MEF2C depletion. As expected, in cells with knockdown of MEF2C, we found mostly downregulated miRNAs; nevertheless, as much as one-third of altered miRNAs were upregulated. The majority of these changes are driven by transcription efficiency. Moreover, we found that MEF2C affects nontemplated 3′-end nucleotide addition of miRNAs, mainly oligouridylation. The rate of these modifications is associated with the level of TUT4 which mediates RNA 3′-uridylation. Finally, we found that a quarter of miRNAs which significantly changed upon differentiation of human skeletal myoblasts is inversely altered in MEF2C deficient cells. We concluded that MEF2C is an essential factor regulating both the quantity and quality of the microtranscriptome, leaving an imprint on the stability and perhaps specificity of many miRNAs during the differentiation of muscle cells. |
| Databáze: | OpenAIRE |
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