TACAN is an essential component of the mechanosensitive ion channel responsible for pain sensing
| Autor: | C Stanton, Emmanuel Bourinet, Amanda MacPherson, U Khan, A Donoghue, Francina Agosti, Alfredo Ribeiro-da-Silva, C Dietz, Tarheen Fatima, Rikard Blunck, Élise Faure, Noosha Yousefpour, Hugues Petitjean, Marine Christin, Reza Sharif-Naeini, Lou Beaulieu-Laroche, Albena Davidova |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Chemistry [SDV]Life Sciences [q-bio] [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Sensory system 03 medical and health sciences 0302 clinical medicine Mechanosensitive ion channel Nociceptor Mechanosensitive channels [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Mechanotransduction Mechanical pain Neuroscience Tactical air navigation system 030217 neurology & neurosurgery Ion channel 030304 developmental biology |
| Popis: | SummaryMechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying several physiological functions such as touch and pain sensing, hearing and proprioception. This process is carried out by specialized mechanosensitive ion channels whose identities have been discovered for most functions except pain sensing. Here we report the identification of TACAN (Tmem120A), an essential subunit of the mechanosensitive ion channel responsible for sensing mechanical pain. TACAN is expressed in a subset of nociceptors, and its heterologous expression increases mechanically-evoked currents in cell lines. Purification and reconstitution of TACAN in synthetic lipids generates a functional ion channel. Finally, knocking down TACAN decreases the mechanosensitivity of nociceptors and reduces behavioral responses to mechanical but not to thermal pain stimuli, without affecting the sensitivity to touch stimuli. We propose that TACAN is a pore-forming subunit of the mechanosensitive ion channel responsible for sensing mechanical pain. |
| Databáze: | OpenAIRE |
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