| Autor: |
Gokul Sudhakaran, Ravi Rajesh, Ajay Guru, B. Haridevamuthu, Raghul Murugan, Nattamai Bhuvanesh, Mohammad Ahmad Wadaan, Shalid Mahboob, Annie Juliet, Pushparathinam Gopinath, Jesu Arockiaraj |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Toxicology and applied pharmacology. 454 |
| ISSN: |
1096-0333 |
| Popis: |
This study aims to evaluate the protective behaviour of N2, a semi-natural analog of nimbin, for its anti-diabetic efficacy against alloxan-induced oxidative damage and β-cell dysfunction in in-vivo zebrafish larvae. A 500 μM of alloxan was exposed to zebrafish larvae for 24 h to induce oxidative stress in the pancreatic β-cells and co-exposed with N2 to study the protection of N2 by inhibiting ROS by DCFH-DA, DHE and NDA staining along with Cellular damage, apoptosis and lipid peroxidation. The zebrafish was further exposed to 500 μM alloxan for 72 h to induce β-cell destruction along with depleted glucose uptake and co-exposed to N2 to study the protective mechanism. Glucose levels were estimated, and PCR was used to verify the mRNA expression of phosphoenolpyruvate carboxykinase (PEPCK) and insulin. Alloxan induced (24 h) oxidative stress in the pancreatic β-cells in which N2's co-exposure inhibited ROS by eliminating O |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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